The potential of <Emphasis Type="Italic">Rhizobium</Emphasis> strains for biological control of <Emphasis Type="Italic">Orobanche crenata</Emphasis> |
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Authors: | Yassine Mabrouk Lazhar Zourgui Bouaziz Sifi Omrane Belhadj |
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Institution: | (1) Laboratoire de Biochimie et de Technobiologie, Faculté des Sciences de Tunis, Elmanar, 2092, Tunisie;(2) Unité de Biochimie Macromoléculaire et Génétique, Faculté des Sciences, 2112 Zarroug Gafsa, Tunisie;(3) Laboratoire des grandes cultures, Institut National de la Recherche Agronomique, rue Hedy karray, 2080 Ariana, Tunisie |
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Abstract: | Orobanche crenata Forsk is a chlorophyll lacking holoparasite that subsists on the roots of plants and causes significant damage to the culture
of leguminous plants and, in particular, to peas (Pisum sativum L.). Here, we investigated the potential of Rhizobium strains for biological control of Orobanche crenata using a commercial pea cultivar (Douce de province) and different Rhizobium strains. Firstly, benefit of bacterial inoculation on plant growth and efficiency in N-incorporation were demonstrated with
four isolates, P.SOM, P.1001, P.Mat.95 and P.1236. After five Rhizobium strains (three efficient: P.SOM, P.1236, P.Mat.95 and two not efficient: P.OM1.92, P.MleTem.92) were investigated for their
ability to control Orobanche crenata using pot and Petri dish experiments. Inoculation of peas with two (P.SOM and P.1236) of the five strains induced a significant
decrease in O. crenata seed germination and in the number of tubercles on pea roots. Furthermore, other symptoms, including the non-penetration
of the germinated seeds into pea roots followed by radicle browning and death of the parasites, were observed in the presence
of these inoculated pea plants. The hypothesis that roots secrete toxic compounds related to Rhizobium inoculation is discussed. |
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Keywords: | biological control Rhizobium strains Orobanche crenata pea necrotic symptoms |
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