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Cellobiohydrolase I (Cel7A) from Trichoderma reesei has chitosanase activity |
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| Authors: | Masakazu Ike Yohane Ko Kengo Yokoyama Jun-Ichi Sumitani Takashi Kawaguchi Wataru Ogasawara Hirofumi Okada Yasushi Morikawa |
| Institution: | aDepartment of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan bDepartment of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan |
| Abstract: | Cellobiohydrolase CBH I (Cel7A) from the filamentous fungus Trichoderma reesei (TrCBHI), which is a member of glycoside hydrolase family (GHF) 7, was expressed in Aspergillus oryzae. We found that the recombinant enzyme showed significant chitosanase activity, as well as cellulase activity, and acted in an endo-type manner on soluble polymeric substrate. Furthermore, another GHF7 CBH I from Aspergillus aculeatus (AaCBHI) expressed in A. oryzae also had chitosanase activity, while endoglucanase EG I (Cel7B) from T. reesei had no activity towards chitosan. To our knowledge, this is the first report of GHF7 enzymes possessing chitosanase activity. |
| Keywords: | Trichoderma reesei (Hypocrea jecorina) Aspergillus aculeatus Cellobiohydrolase Chitosanase Glycoside hydrolase family (GHF) 7 |
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