Aquaporins in developing rice grains |
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| Authors: | Hidehiro Hayashi Junko Ishikawa-Sakurai Mari Murai-Hatano Arifa Ahamed Matsuo Uemura |
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| Institution: | 1. Agro-Production Technologies and Management Research Division, NARO Tohoku Agricultural Research Center, Morioka, Japan;2. United Graduate School of Agricultural Sciences, Iwate University, Morioka, Japanhdhrhys@affrc.go.jp;4. Faculty of Agriculture, Cryobiofrontier Research Center, Iwate University, Morioka, Japan;5. United Graduate School of Agricultural Sciences, Iwate University, Morioka, Japan;6. Faculty of Agriculture, Cryobiofrontier Research Center, Iwate University, Morioka, Japan |
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| Abstract: | During rice grain filling, grain moisture content and weight show dynamic changes. We focused on the expression of all 33 rice aquaporins in developing grains. Only two aquaporin genes, OsPIP2;1 and OsTIP3;1, were highly expressed in the period 10–25 days after heading (DAH). High-temperature treatment from 7 to 21 DAH abolished the dynamic up-regulation of OsPIP2;1 in the period 15–20 DAH, whereas OsTIP3;1 expression was not affected. Immunohistochemical analysis revealed that OsPIP2;1 was present in the starchy endosperm, nucellar projection, nucellar epidermis, and dorsal vascular bundles, but not in the aleurone layer. OsTIP3;1 was present in the aleurone layer and starchy endosperm. Water transport activity of recombinant OsTIP3;1 was low, in contrast to the high activity of recombinant OsPIP2;1 we reported previously. Our data suggest that OsPIP2;1 and OsTIP3;1 have distinct roles in developing grains. |
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| Keywords: | aleurone layer aquaporin developing grain Oryza sativa phloem |
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