Human complement subcomponent C2: purification and proteolytic cleavage in fluid phase by C1s, C1r2-C1s2 and C1 |
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Authors: | N M Thielens M B Villiers A Reboul C L Villiers M G Colomb |
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Affiliation: | Equipe de Recherche ‘Immunochimie — Système Complémentaire’ du DRF-G et USM-G, Associée au CNRS (ERA no. 695) et à l''INSERM (U. no. 238), Laboratoire de Biologie Moléculaire et Cellulaire, Centre d''Etudes Nucléaires de Grenoble, 85 X, 38041 Grenoble Cédex, France |
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Abstract: | ![]() Photosynthetic membranes contain considerable regions of high surface curvature, notably at their margins, where the average radius of curvature is about 10 nm. The proportion of total membrane lipid in the outer and inner thylakoid margin monolayers is estimated at 21% and 13%, respectively. The major thylakoid lipid, monogalactosyldiacylglycerol, is roughly cone-shaped and will not form complete lamellar bilayer phases, even in combination with other thylakoid lipids. It is proposed that this galactolipid plays a role in: (a) stabilising regions of concave curvature in thylakoids; and (b) packaging hydrophobic proteins in planar bilayer regions by means of inverted micelles. This model predicts substantial asymmetries in the distribution of lipids both across and along the thylakoid bilayer plane. |
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Keywords: | SDS—PAGE sodium dodecyl sulphate polyacrylamide gel electrophoresis TEA triethanolamine EDTA ethylene diamine tetra-acetic acid DFP diisopropylphosphorofluoridate Ig immunoglobulin C4bp C4-binding protein |
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