| 噬菌体表面呈现技术研究进展 |
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| 引用本文： | 冯东晓,李敏,李春海.噬菌体表面呈现技术研究进展[J].生物技术通讯,1997(1). |
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| 作者姓名： | 冯东晓 李敏 李春海 |
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| 作者单位： | 军事医学科学院附属医院肿瘤分子生物学室，军事医学科学院附属医院肿瘤分子生物学室，军事医学科学院附属医院肿瘤分子生物学室 北京 100850，北京 100850，北京 100850 |
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| 摘 要： | 噬菌体表面呈现技术是1985年建立的一种将外源基因表达呈现在噬菌体颗粒表面的方法,可用于建立随机多肽文库、抗体文库等。经特定配基的筛选,可获得与其特异结合的配体分子。通过改构,还可将cDNA产物表达于噬菌体颗粒的尾部构建cDNA文库。SIP技术通过将配体和配基分别与基因Ⅲ蛋白的C末端和N末端融合表达,基因Ⅲ的C-末端参与噬菌体颗粒的组装,配基与配体的结合能够重建基因Ⅲ蛋白的功能,才能形成有感染能力的噬菌体,这样就大大提高了筛选效率。
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| 关 键 词： | 噬菌体表面呈现文库 基因Ⅲ蛋白 选择性感染噬菌体技术 |
Filamentous phage surface display |
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| Feng Dongxiao,Li Min,Li Chunhai.Filamentous phage surface display[J].Letters in Biotechnology,1997(1). |
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| Authors: | Feng Dongxiao Li Min Li Chunhai |
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| Abstract: | Filamenlous phage surface display was a method that firstly established by Smith in 1985 which can display foreign gene on the surface of the phage particle. Several kinds of libraries such as random polypeptides libraries, antibody libraries have been established based on this method . The libraries can be screened by affinily hgand such as antibody. cDNA libraries can also be established by improved method. Selective infective phage (SIP) method is the newly esablished screening method which can greatly improved the sereening efficiency. |
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| Keywords: | filamenlous phage surface display libraries gene 3 protein selective infective phage |
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