Diverse sugar-binding specificities of marine invertebrate C-type lectins |
| |
Authors: | Matsubara Hiroki Nakamura-Tsuruta Sachiko Hirabayashi Jun Jimbo Mitsuru Kamiya Hisao Ogawa Tomohisa Muramoto Koji |
| |
Institution: | Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University, Sendai, Japan. |
| |
Abstract: | The sugar-binding specificities of C-type lectins isolated from marine invertebrates were investigated by frontal affinity chromatography (FAC) using 100 oligosaccharides. The lectins included BRA-2 and BRA-3, multiple lectins from the hemolymph of the acorn barnacle, Megabalanus rosa, and BRL from the acorn barnacle, Balanus rostatus. The diverse sugar-binding specificities of the C-type lectins were determined by FAC analysis. BRA-2 recognized alpha2-6 sialylation but not alpha2-3 sialylation on glycans. On the other hand, BRA-3 showed high affinity for oligosaccharides with alpha-linked non-reducing terminal galactose, but not for sialylated forms, and BRL showed enhanced recognition activity towards Lewis(x) and Lewis(a) epitopes. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|