Phenol degradation and colour removal in submerged culture of Pleurotus sajor-caju with paper mill effluents |
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Institution: |
a Instituto de Biotecnologia - Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil |
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Abstract: | The fungus Pleurotus sajor-caju secretes phenol-oxidases that enable the use of recalcitrant compounds as substrates. The residues of paper manufacture contain high lignin levels, which gives the effluents a characteristic brownish colour. To test the potential of P. sajor-caju cultures on reducing these parameters, we used 90% of raw effluents from medium consistency oxygen delignification and bleaching stages plus 10% of mineral solution and different levels of glucose (5-15 g L-1) as substrate. We observed a greater fungal biomass in cultures using effluent than in controls. Cultures containing 10 to 15 g L-1 of glucose resulted in about 42% colour reduction. The polyphenol content was also reduced by 58.9% by the 13th day of culture. In addition, we observed the secretion of laccases (211.44 U mL-1 and 45.98 U mL-1 using ABTS and syringaldazine, respectively) and peroxidases (6.11 U mL-1-ABTS) both peaking at the 7th day of culture and with similar kinetics of production in different glucose concentrations. |
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Keywords: | Biodegradation laccase paper mill peroxidase Phenol Pleurotus sajor-caju |
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