A new system for the expression of human interleukin-6 inEschrichia coli |
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Authors: | Kiyoshi Yasukawa Takashi Saito |
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Institution: | (1) Biotechnology Research Laboratory, TOSOH Corporation, 2743-1, Hayakawa, 252 Ayase-shi, Kanagawa-ken, Japan |
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Abstract: | Summary We constructed a new type of plasmid from which peptides containing interleukin-6 (IL-6) were produced inE. coli as human growth hormone (hGH) fusion proteins. The products were cleaved by thrombin to liberate IL-6, which was purified to homogeneity. |
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