黄连须和小檗碱对草鱼非特异性免疫系统的影响及对嗜水气单胞菌感染的抵抗作用

为了评估黄连须(FRC)和小檗碱(BBR)对草鱼(Ctenopharyngodon idella)的非特异性免疫及对抵抗嗜水气单胞菌感染的影响, 鱼喂食含1.00%、0.50%、0.25%, 0.10%, 0.00%的FRC和0.05%BBR的饲料28d。喂养28d后, 鱼注射嗜水气单胞菌, 记录感染14d后的存活率。检测NBT活性、血清溶菌酶活性、吞噬活性和补体C3水平来评价药物对草鱼的免疫反应。结果表明, 与模型组相比, 喂养饲料含有FRC和BBR的各组提高NBT活性、血清溶菌酶活性、吞噬活性和草鱼补体C3水平。喂食0.50%FRC组感染细菌后的存活率最高, 与模型组比较增加44%。FRC和BBR的体外抗菌活性采用最小抑菌浓度的方法进行了研究, 并显示出较强的抑制作用。结果表明, FRC和BBR可以用作免疫刺激剂以提高草鱼非特异性免疫和对嗜水气单胞菌引起疾病的抵抗。  

EFFECT OF FIBROUS ROOT OF COPTIS CHINENSIS FRANCH AND BERBERINE ON THE NON-SPECIFIC IMMUNITY AND RESISTANCE AGAINST AEROMONAS HYDROPHILA INFECTION IN GRASS CARP (CTENOPHARYNGODON IDELLA)

To determine the effects of Fibrous root of Coptis chinensis Franch (FRC) and berberine (BBR) on non-specific immunity and disease resistance against Aeromonas hydrophila infection in grass carp (Ctenopharyngodon idella), fish were fed with diets containing 1.00%, 0.50%, 0.25%, 0.10%, 0.00% of FRC powder and 0.05% of BBR for 28 days. Fish were challenged with A. hydrophila 28 days post feeding and survival rate (SR) was recorded over 14 days post challenge. Nitroblue tetrazolium (NBT) activity, serum lysozyme activity, phagocytic activity and complement C3 level were studied to assess the immune response of fish. The results revealed that dietary FRC and BBR increase the NBT activity, serum lysozyme activity, phagocytic activity and complement C3 level in grass carp when compared to the model group (MD). Feed containing 0.50% FRC was the most effective with the SR of the fish significantly increased by 44% compared to MD. The in vitro antibacterial activity of FRC and BBR were investigated with the minimum inhibitory concentration (MIC) method and showed strong inhibitory effects. The results indicated that FRC and BBR can be used as immunostimulants to enhance the non-specific immunity and disease resistance of grass carp against A. hydrophila.