温度胁迫对白车轴草水浸提液化感作用的影响

采用蚕豆根尖细胞微核试验和染色体畸变试验,研究了温度胁迫下白车轴草水浸提液对蚕豆根尖细胞的化感作用。结果表明:不同温度(5℃、25℃和35℃)处理后的白车轴草的水浸提液对蚕豆根尖细胞有丝分裂指数的影响为低浓度促进,高浓度抑制。在白车轴草水浸提液的作用下,蚕豆根尖细胞内出现了多种染色体畸变,如微核、染色体断片、染色体桥、落后染色体等,对微核率和染色体畸变率的诱导效应表现为随水浸提液浓度的增大而增大。温度胁迫后的化感作用明显大于胁迫前,其化感效应表现为低温(5℃)下白车轴草水浸提液高温(35℃)常温(25℃)白车轴草水浸提液。推测原因可能是温度胁迫改变和增加了化感物质的释放。

Temperature Influences on the Allelopathy Effect of Aqueous Extracts from Trifolium repens L.

The allelopathy effect of aqueous extracts from Trifolium repens L. on root tip cells of Vicia faba under different temperature (5℃,25℃ and 35℃) stresses was investigated by using micronucleus assay and chromosome aberration assay. The results indicated that the mitotic index of V.faba root tip cells treated by low concentration of aqueous extracts increased and it decreased at high concentration, the aqueous extracts of T.repens also caused various types of chromosome aberrations such as micronucleus, chromosome fragment, chromosomal bridge and lagging chromosome,and micronucleus and aberration rates increased along with concentration of extracts. This research showed the allelopathy effects of aqueous extracts from T.repens by different temperature treatments were different, the low temperature group (5℃) was the strongest, high temperature group (35℃) was second, normal temperature（25℃）was the smallest, which was probably due to temperature stress changed and increased the secondary metabolites of T.repens, increased genetic toxicity of the aqueous extracts of this plant.