Independent regulation of serologically distinct idiotypes in F1 hybrid mice |
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Authors: | Thomas F Kresina Mitchell J Nelles Alfred Nisonoff Alan R Brown |
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Institution: | (1) Rosenstiel Research Center and Department of Biology, Brandeis University, 02254 Waltham, Massachusetts;(2) The Division of Immunology, St. Jude Children's Research Hospital, 38101 Memphis, Tennessee;(3) Rosenstiel Research Center, Brandeis University, 02254 Waltham, MA |
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Abstract: | We have investigated the regulation of expression of two distinct intrastrain cross-reactive idiotypes, CRIA and CRIC, characteristic of anti-p-azophenylarsonate (anti-Ar) antibodies of the A/J and BALB/c strains, respectively, in (BALB/c x A/J)F1 (CAF1) mice. Such hybrid mice were found to synthesize antibodies with each idiotype when immunized against the Ar hapten group, although the expression of each was significantly reduced as compared with the parental strain. CAF1 mice were pretreated with idiotypic-specific antibody reagents and subsequently hyperimmunized against the Ar hapten. Analysis of the idiotypes present in immune sera showed that suppression of either CRI did not concomitantly suppress the expression of the other. Alteration of the expression of one idiotype was not, however, without influence on the other; the expression of CRIC was markedly enhanced in mice suppressed for CRIA.Abbreviations used in this paper anti-Id(A/J)
idiotypic-specific antibodies against A/J serum Ar-specific antibodies
- anti-Id(BALB/c)
idiotypic-specific antibodies against BALB/c serum Ar-specific antibodies
- Ar
p-azophenylarsonate
- BGG
bovine -globulin
- BSA
bovine serum albumin
- CAF1
F(BALB/c x A/J)
- CFA
complete Freund's adjuvant
- CRIA
the major cross-reactive idiotype of A/J Ar-specific antibodies
- CRIC
the major cross-reactive idiotype of BALB/c Ar-speck antibodies
- CRIm
the minor cross-reactive idiotype of A/J Ar-specific antibodies
- DTH
delayed-type hypersensitivity
- HP
hybridoma product(s)
- KLH
keyhole limpet hemocyanin |
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