菲菊头蝠冬眠期的精子储存

菲菊头蝠是分布于亚热带和热带的翼手目种类,在中国大陆南部和海南岛有广泛分布。为探讨热带菲菊头蝠是否具有冬眠期(12 月至翌年2 月)储精现象及生殖腺组织结构的变化,对海南岛的菲菊头蝠成年雄蝠和成年雌蝠生殖系统在冬眠期间的变化进行了石蜡切片观察。结果显示:成年雄蝠在冬眠期间附睾内储存大量精子,推测其储存时间超过2 个月;冬眠期间曲细精管横截面积、精子细胞数量和间质细胞数量在冬眠期逐月显著性减少,精原细胞和精母细胞的数量在12 月与翌年1 月间均无显著性变化,而在冬眠末期的2 月显著增多。在雌蝠子宫和卵巢内均未发现精子储存现象,但卵巢内具有初级卵泡和次级卵泡,雄蝠在冬眠期间曲细精管逐月萎缩,但生精上皮精母细胞的数量在冬眠末期明显上升。

Sperm storage in least horseshoe bat during hibernation on Hainan Island

Least horseshoe bat (Rhinolophus pusillus) occurs in subtropical and tropical regions including the southern part of the mainland and Hainan Island in China. In order to examine sperm storage in least horseshoe bat during hibernation in Hainan Island,we collected adult male and adult female least horseshoe bats in hibernation to observe sperm storage in testis and epididymis of males,and ovary and uterus of females. During the hibernation,males stored sperm in epididymis for more than two months from December to February. The cross sectional areas ofeminiferous tubules,spermatids, and leydig cells significantly decreased. Spermatogonium and spermatocyte did not significantly change between December and January,but significantly increased in February. However,female bats did not store sperm in their ovary and uterus. The seminiferous tubule of male bat shrank during hibernation but spermatogonium of spermatogenic epithelium increased significantly at the end of hibernation. Primary and secondary follicles were found in the ovaries of female bats during hibernation.