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Isolation and expansion of equine umbilical cord-derived matrix cells (EUCMCs) |
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| Authors: | Passeri Simona Nocchi Francesca Lamanna Roberta Lapi Simone Miragliotta Vincenzo Giannessi Elisabetta Abramo Francesca Stornelli Maria Rita Matarazzo Micheletino Plenteda Daniele Urciuoli Patrizia Scatena Fabrizio Coli Alessandra |
| Institution: | a Department of Veterinary Anatomy, Biochemistry and Physiology, University of Pisa, Pisa, Italy b Cell Biology and Tissue Regeneration Laboratory-Immunohaematology 2 Unit - Azienda Ospedaliera Universitaria Pisana, Pisa, Italy c Department of Animal Pathology, University of Pisa, Pisa, Italy d Centro Militare Veterinario of Grosseto, Grosseto, Italy |
| Abstract: | Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy.EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in α-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to α-MEM.α-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of α-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol.Both α-MEM and MSCBM can be used to cultivate EUCMCs. α-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since α-MEM is more cost-effective than MSCBM. |
| Keywords: | Umbilical cord-derived matrix cells Umbilical cord Stem cells Equine Epidermal growth factor |
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