Refixation of photorespiratory ammonia and the role of alanine in photorespiration: Studies with15N |
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Authors: | Betsche Thomas Eising Rainer |
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Affiliation: | (1) Botanisches Institut der Universität Münster, Schlossgarten 3, D-4400 Münster, FRG |
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Abstract: | Summary Labelling experiments with15N glutamate and15N alanine were conducted using slices from oat leaves to investigate photorespiratory nitrogen metabolism. It is concluded from the labelling kinetics of glutamine that the refixation of photorespiratory ammonia primarily occurs by glutamine synthetase in the chloroplast. The labelling kinetics of glutamine with15N glutamate indicate that the chloroplastic and cytoplasmic glutamate pools do not exchange easily in oat leaf cells. Alanine was shown to be an important amino donor for photorespiratory glycine formation. This result is discussed with regard to a possible role of alanine in photorespiration. A modification to the scheme of photorespiratory nitrogen metabolism is proposed. |
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Keywords: | Alanine Aminotransferases Ammonia refixation Avena sativa L. Glycine Membranes Oat Peroxisomes Photorespiration |
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