Effects of morphine on dopamine-stimulated adenylate cyclase and on cyclic GMP formation in primate brain amygdaloid nucleus. |
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Authors: | D Wilkening R K Mishra M H Makman |
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Institution: | Departments of Pharmacology and Biochemistry Albert Einstein College of Medicine, Bronx, N.Y.USA 10461 |
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Abstract: | In homogenates of (Rhesus) or amygdaloid nuclear complex, adenylate cyclase activity was approximately doubled by either 10μ dopamine or 8m NaF. In the presence of morphine, the stimulation by dopamine was reduced. A 90–100% inhibition of the dopamine stimulation was obtained with 20μ, and a 50% inhibition, with 5μ morphine. The effects of 10μ morphine on dopamine stimulation were reversed by 10μ naloxone. Morphine itself did not significantly affect the basal adenylate cyclase activity, but in the presence of 10μ morphine the stimulation by 8m NaF was reduced approxiamtely 50%. The data suggest an action of morphine at a receptor site which is distinct from the dopamine receptor, but which inhibits the dopamine-stimulated adenylate cyclase. In addition, the cyclic GMP content of amygdala slices was reduced by 50–75% during incubation for 5–20 minutes with morphine. Maximum effects on cyclic GMP were obtained with 10μ, and half-maximum effects, with 0.1μ morphine. The effect of morphine on amygdala cyclic GMP was not reversed by naloxone. Thus, this action of morphine may not be receptor mediated, or may involve the interaction of morphine with receptors other than the opiate receptor. |
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