Hydroxysteroid dehydrogenase of Cochliobolus lunatus |
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| Affiliation: | 1. University of Applied Sciences and Arts Northwestern Switzerland, School of Life Sciences, Gründenstrasse 40, CH-4132 Muttenz, Switzerland;2. University of Basel, Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, Klingelbergstrasse 50, 4056 Basel, Switzerland;3. IRCCS—Istituto di Ricerche Farmacologiche “Mario Negri”, Environmental Biomarkers Unit, Department of Environmental Health Sciences, Via La Masa 19, I-20156 Milan, Italy;4. Swiss Federal Institute of Technology (ETH Zürich), Institute of Biogeochemistry and Pollution Dynamics, Department of Environmental System Sciences, CH-8092 Zürich, Switzerland;1. MTA-ÖK BLI NAP_B Adaptive Neuroethology, Department of Experimental Zoology, Balaton Limnological Institute, MTA Center for Ecological Research, Tihany, Hungary;2. Department of Chemistry, W. M. Keck Institute for Proteomics Technology and Applications, The George Washington University, WA, District of Columbia 20052, USA;3. MTA-ÖK BLI, Department of Experimental Zoology, Balaton Limnological Institute, MTA Center for Ecological Research, Tihany, Hungary;1. School of Life Sciences, Guangzhou University, Guangzhou 510655, China;2. Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;3. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;4. The Environmental Research Institute, MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China;1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China;2. School of Environment, Northeast Normal University, Changchun 130117, Jilin, PR China |
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| Abstract: | ![]()
Cochliobolus lunatus is known to be able to hydroxylate steroids at position 11β. Besides this inducible enzyme, we found a constitutive hydroxysteroid dehydrogenase activity which is strongly regioselective with the highest activity at position 17, and the best substrate was found to be androstenedione. Using different substrates, no such activities were observed at positions 3 or 11. The enzyme is membrane bound and NADH or NADPH dependent. The protoplasts of Cochliobolus lunatus show the same activity as intact cells, which means that the cell wall does not influence the reaction. |
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