Characterization of single chain antibody targets through yeast two hybrid |
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| Authors: | Ole Vielemeyer Clément Nizak Ana Joaquina Jimenez Arnaud Echard Bruno Goud Jacques Camonis Jean-Christophe Rain Franck Perez |
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| Affiliation: | 1.Institut Curie - Research Center,Paris cedex 05,France;2.CNRS, UMR144,Paris, cedex 05,France;3.U830 INSERM,Paris cedex 05,France;4.Hybrigenics Inc,Paris,France;5.Drexel University School of Medicine,Division of Infectious Diseases &HIV Medicine,Philadelphia,USA;6.Université Joseph Fourier (Grenoble) Laboratoire de Spectrométrie Physique, CNRS,Saint Martin d'Hères,France;7.Institut Pasteur, Membrane Traffic and Cell Division Lab,Paris,France |
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| Abstract: | Background Due to their unique ability to bind their targets with high fidelity, antibodies are used widely not only in biomedical research, but also in many clinical applications. Recombinant antibodies, including single chain variable fragments (scFv), are gaining momentum because they allow powerful in vitro selection and manipulation without loss of function. Regardless of the ultimate application or type of antibody used, precise understanding of the interaction between the antibody's binding site and its specific target epitope(s) is of great importance. However, such data is frequently difficult to obtain. |
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