Isolation and structure of phage lambda head-mutant DNA |
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| Authors: | R G Wake A D Kaiser R B Inman |
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| Affiliation: | Department of Biochemistry, Stanford University Stanford, Calif. 94305, U.S.A.; Biophysics Laboratory and Biochemistry Department University of Wisconsin, Madison, Wis. 53706, U.S.A. |
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| Abstract: | High molecular weight DNA accumulates in bacteria in which λ is multiplying but cannot complete the formation of new phage particles due to a defect in head assembly. Accumulated λ DNA has been isolated from induced mitomycin C-treated lysogens by means of a shift in buoyant density labels from heavy to light and fractionation by density-gradient sedimentation for completely light DNA. Head formation was blocked in these lysogens by amber mutations in genes D or E, which specify the two major head proteins. The purified DNA is at least 80% λ by DNA-DNA hybridization and some preparations are close to 100% λ by this test. |
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