<Emphasis Type="Italic">Synechocystis</Emphasis> PCC 6803 cells heterologously expressing bacterial tyrosine ammonia lyase can use exogenous tyrosine for <Emphasis Type="Italic">p</Emphasis>-coumaric acid production |
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| Authors: | S Tantong N Nuengchamnong S Kumphune A Incharoensakdi P Lindblad S Sirikantaramas |
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| Institution: | 1.Program in Biotechnology, Faculty of Science,Chulalongkorn University,Bangkok,Thailand;2.Department of Biochemistry, Faculty of Science,Chulalongkorn University,Bangkok,Thailand;3.Science Laboratory Centre, Faculty of Science,Naresuan University,Phitsanulok,Thailand;4.Department of Medical Technology, Faculty of Allied Health Sciences,Naresuan University,Phitsanulok,Thailand;5.Microbial Chemistry, Department of Chemistry –?ngstr?m,Uppsala University,Uppsala,Sweden;6.Omics Sciences and Bioinformatics Center, Faculty of Science,Chulalongkorn University,Bangkok,Thailand |
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| Abstract: | Phototrophic cyanobacteria may be considered as an alternative host for producing numerous bioactive compounds. We demonstrate that the Synechocystis PCC 6803 expressing tyrosine ammonia-lyase from Rhodobacter sphaeroides under Ptrc1O promoter produce p-coumaric acid at a rate three times higher than that under Ptrc1Ocore promoter, accounting for 18.4 ± 1.5 μg of p-coumaric acid per 108 cells (0.36 mg L?1). Additionally, our study is the first report to show the biotransformation of tyrosine to p-coumaric acid reaching a maximum 2.4-fold increase when 0.5 mM tyrosine was supplemented to the growth medium. Liquid chromatography-mass spectrometry analysis revealed the occurrence of diverse patterns of metabolites under different concentrations of supplemented tyrosine, suggesting that it is used in additional metabolic pathways. |
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