Inhibition of ovulation in rabbits by intrafollicular injection of indomethacin and prostaglandin F antiserum |
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Authors: | D T Armstrong D L Grinwich Y S Moon J Zamecnik |
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Institution: | Dept. of Obstetrics & Gynecology, and Dept. of Physiology, University of Western Ontario, London, Ontario, Canada |
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Abstract: | Indomethacin, an inhibitor of prostaglandin biosynthesis, was 100% effective in blocking luteinizing hormone (LH)-induced ovulation when administered via microinjection directly into 22 follicles in twelve rabbits, 5 hours after intravenous injection of the gonadotropin. Ovulation was similarly blocked in 24 of 25 follicles injected with antiserum prepared against prostaglandin F2α. Antiserum against prostaglandin E2, at the same dosage (100 μg lyophilized serum per follicle), was considerably less effective, preventing ovulation in only 6 of 14 follicles. Control follicles injected with the phosphate buffer vehicle, or with normal rabbit serum, underwent normal ovulation and luteinization. LH injection caused a striking increase in concentration of F-type prostaglandins in follicles shortly before ovulation, an increase which was prevented by i.v. or intrafollicular injection of ovulation-blocking blocking dosages of indomethacin. These findings provide evidence in support of a role for prostaglandins, acting at the follicular level, in the process of ovulation. |
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