Plant regeneration from tissue cultures initiated from immature inflorescences of a grass,Echinochloa colonum (L.) link |
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| Authors: | A K Tyagi S Bharal A Rashid N Maheshwari |
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| Institution: | (1) Unit for Plant Cell and Molecular Biology and Department of Botany, University of Delhi, 110007 Delhi, India;(2) Present address: Botanisches Institut der Universität Düsseldorf, Universitatstrasse 1, D-4000 Düsseldorf 1, FRG;(3) Present address: Gargi College, University of Delhi, Siri Fort Road, 110049 New Delhi, India |
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| Abstract: | Organised structures develop on a white and compact callus initiated from small segments of immature inflorescences ofEchinochloa colonum cultured on Murashige and Skoog's (MS) medium supplemented with 5.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 10% coconut milk. These develop into plantlets upon subculture onto MS medium containing 0 or 0.2 mg/l 2,4-D. Twelve out of 17 plantlets regenerated grew well on transfer to soil and eleven plants produced seeds. |
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