八株芽孢杆菌菌株的分类及固氮活性的测定

从8个省市的不同土壤分离了200个菌株，筛选出8株（JR₁，JR₂，JR₅，ZZ₃，ZZ₁₂，ZZ₁₆，M₁，K₄）能在无氮培养基上生长良好的典型的芽抱杆菌，其中6株（JR₁，JR₂，JR₄和ZZ₃，ZZ₁₂，ZZ

THE ISOLATION IDENTIFICATION AND DETERMINATION OF THE NITROGEN-FIXING ABILITIES OF EIGHT BACILLUS STRAINS

The soils from Beijing municipality and seven provinces in Chinese were examined for the incidence of Bacillus nitrogen-fixer. Only six strains of tWo hundreds isolated strains were able to reduce acetylene. Eight strains which grew well on nitrogen-free medium According to the morphological and thirty-six biochemical characteristics and G Cmol%,three strains(JR1, JR2, JR5) were identified as Bacillus azotofixans, but were different to four biochemical tests from ATCC 35681; three strains(ZZ3, ZZ12, ZZ16) were different from two type strains on morphological and biochemical tests, eleven characteristics it from ATCC 35681,seven tests from ATCC 842; M1 strain was identified as Bacikllus polymyxa, because it's taxonomical characteristics were consistent with ATCC 842; K4 strain was deferent from ATCC 35681 on which fermentation of carbohydrates,hydrolysis of starch and casin, resistance to salt. The growth curve of JR1 strain was identical to ATCC 35681, while the lag period of ZZ12 strain was shorter and culture solution was turbier.