The effect of epidermal growth factor on chronotropic response in cardiac cells in culture |
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| Authors: | S W Rabkin P Sunga S Myrdal |
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| Affiliation: | 1. Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Louisville, KY 40202, USA;2. Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USA;3. Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Louisville School of Medicine, Louisville, KY 40202, USA;4. The Robley Rex Veterans Affairs Medical Center, Louisville, KY 40206, USA;5. The Jewish Hospital Liver Transplant Program, Louisville, KY 40202, USA;6. The Proteomics Core, University of Louisville School of Medicine, Louisville, KY 40202, USA;7. Hepatobiology & Toxicology Center, University of Louisville School of Medicine, Louisville, KY 40202, USA;8. University of Louisville Alcohol Research Center, University of Louisville, Louisville, KY 40202, USA;9. University of Louisville Superfund Research Program, University of Louisville, Louisville, KY 40202, USA |
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| Abstract: | Cardiac chronotropic response to epidermal growth factor (EGF) was assessed in chick embryonic ventricular cell aggregates. EGF at a concentration of 10 ug/mL but not at 5 ug/mL produced a significant (p less than 0.05) increase in cardiac beating rate. This was evident within 10 min, reached a peak at about 15 min and remained at that level for 1.5 hr or the rest of the observation period. The effect of EGF on cardiac automaticity was reduced but not abolished at a lower temperature (22oC) that is known to decrease the affinity of the EGF receptor and reduce the internalization of EGF. Hypothermia did not change the maximum increase in heart rate response from isoproterenol although it altered the pattern of the response. Beta adrenoreceptor blockade with metoprolol only slightly altered the response to EGF. These data indicate that EGF produces functional effects on the heart that may be mediated through EGF receptor linked mechanisms. |
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