Histochemical localization of ascorbic acid in the testis, epididymis and vas deferens of some rodents

Histochemical localization of ascorbic acid was carried out in the testis, epididymis and vas deferens of rat, guinea pig, mouse and also human beings, using a modified technique (CHINOY and SANJEEVAN 1978). The staining pattern was same in all cases, wherein, the nuclei were stained more intensely as compared to the cytoplasm. The luminal spermatozoa were also darkly stained. The significance of the localization is discussed in the light of the recent findings.