Interaction of cyanine dyes with nucleic acids: XXXI. Using of polymethine cyanine dyes for the visualization of DNA in agarose gels |
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| Authors: | Matselyukh B P Yarmoluk S M Matselyukh A B Kovalska V B Kocheshev I O Kryvorotenko D V Lukashov S S |
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| Institution: | Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 154 Zabolotny Street, Kyiv 03143, Ukraine. bohdan@serv.imv.kiev.ua |
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| Abstract: | Fifteen polymethine cyanine dyes were studied as fluorescent stains for DNA in electrophoretic gels. Among studied cyanines, two dyes CPent V and CCyan 2-O most effectively visualized covalently closed and linear double-stranded DNA molecules in gels under standard conditions using UV-illumination, green filter and black-and-white photo film. Ethidium bromide was 1.2-1.6 times more effective as compared to cyanine dyes in staining of DNA in the concentration range of 8-18 ng, while studied cyanines were more sensitive to DNA quantity above 50 ng. |
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