| 冬虫夏草菌液体培养补料对菌丝体和腺苷产量的影响 |
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| 引用本文： | 李潇英,姚一建.冬虫夏草菌液体培养补料对菌丝体和腺苷产量的影响[J].菌物学报,2015,34(5):1015-1023. |
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| 作者姓名： | 李潇英 姚一建 |
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| 作者单位： | 1中国科学院微生物研究所真菌学国家重点实验室 北京 1001012中国科学院大学 北京 100049 |
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| 基金项目： | 青海省科技计划项目（2014-NS-525） |
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| 摘 要： | 为提高冬虫夏草菌菌丝体及腺苷产量,探索了液体培养中进行营养物质补料的方法。通过在培养10d时进行碳、氮源联合补料,将浓度分别补充至47g/L和0.76g/L,则冬虫夏草菌丝体干重和腺苷产量分别达到27.94g/L和1.15mg/g（20d培养周期）,为对照组的2.43倍和19.2倍。这项研究对冬虫夏草菌的大规模工业应用有参考意义。
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| 关 键 词： | 补料 菌丝体积累 次级代谢产物 |
| 收稿时间： | 2015-01-28 |
Fed-batch fermentation of Ophiocordyceps sinensis for the production of mycelia and adenosine |
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| Authors: | Xiao-Ying LI Yi-Jian YAO |
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| Institution: | 1State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China2University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Fed-batch fermentation process for the production of mycelia and adenosine in Ophiocordyceps sinensis, one of the best known traditional Chinese medicines, was developed. The feeding of mixed carbon and nitrogen sources into the liquid culture on the tenth day, raising their concentrations to 47g/L and 0.76g/L respectively, was found to produce 27.94g/L of dry mycelial weight and 1.15mg/g of adenosine after 20d of culture, reaching 2.43 and 19.2 times of that of the control respectively. The results of this study may provide useful information for large-scale fermentation of O. sinensis. |
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| Keywords: | fed-batch fermentation mycelial growth secondary metabolite |
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