Effect of anaerobiosis on alcohol dehydrogenase in oat seedlings |
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Authors: | Hisashi Kato-Noguchi |
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Institution: | (1) Department of Biochemistry and Food Science, Faculty of Agriculture, Kagawa University, 761-0795 Miki, Japan |
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Abstract: | In order to clarify the induction of alcohol dehydrogenase (ADH) by anaerobiosis in oat (Avena sativa L.), the seedlings were exposed to anaerobiosis and activity of ADH and ADH isozyme profiles were determined. The anaerobiosis
increased ADH activities in shoots and roots of the seedlings. By day 2, the activity increased 5 and 4 times in the roots
and the shoots, respectively, compared with those under aerobic condition. Based on nondenaturing electrophoresis, ADH isozyme
composition analysis revealed six bands consisting of a dimmer enzyme with submits encoded by three different Adh genes. Changes in staining intensity of the isozymes indicated that the increase in ADH activity in oat under anaerobiosis
resulted from increased enzyme synthesis. |
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Keywords: | Alcohol dehydrogenase anaerobiosis Avena sativa flooding ADH isozyme oat waterlogging |
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