| 猪大肠杆菌耐热肠毒素和热敏肠毒素基因的融合及表达 |
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| 引用本文： | 王嘉福,陆承平.猪大肠杆菌耐热肠毒素和热敏肠毒素基因的融合及表达[J].微生物学报,2003,43(1):43-47. |
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| 作者姓名： | 王嘉福 陆承平 |
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| 作者单位： | 1. 南京农业大学动物医学院,南京,210095;贵州大学生物与环境科学学院,贵阳,550025
2. 南京农业大学动物医学院,南京,210095 |
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| 基金项目： | 国家自然科学基金资助 ( 39660 0 0 3) |
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| 摘 要： | 用聚合酶链反应扩增出猪源大肠杆菌编码ST前体（proST）和LT的B亚单位(LTB)成熟多肽的序列，再通过套式PCR将proST编码序列3′端和LTB编码序列5′端融合，并置于同一阅读框内，得到ST和LTB的融合基因，将此序列克隆到pGEMT质粒中，序列分析后，亚克隆到表达载体pQE30中，在大肠杆菌细胞中得到表达，表达的融合蛋白同时具有ST和LTB的抗原性，且无ST和LT的生物毒性。
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| 关 键 词： | 猪大肠杆菌，肠毒素，基因融合 |
| 文章编号： | 0001-6209(2003)01-0043-05 |
The Fusion and Expression of Genes Encoding Heat-stable and Heat-labile Enterotoxins of Escherichia coli from Porcine Origin |
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| Wang Jiafu , Lu Chengping.The Fusion and Expression of Genes Encoding Heat-stable and Heat-labile Enterotoxins of Escherichia coli from Porcine Origin[J].Acta Microbiologica Sinica,2003,43(1):43-47. |
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| Authors: | Wang Jiafu Lu Chengping |
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| Institution: | College of Veterinary medicine, Nanjing Agricultural University, Nanjing 210095, China. jiaff@hotmail.com |
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| Abstract: | The genes encoding precursor heat-stabile (pro-ST) and mature peptide of B unit of heat-labile (LT) enterotoxins of Escherichia coli from piglet were amplified by polymerase chain reaction (PCR). The 3' terminus of gene encoding pro-ST was genetically fused to the 5' terminus of the LTB subunit gene in nest-PCR. The fusion genes encoding pro-ST and LTB were cloned into pGEM-T vector and subcloned into the pQE30. The recombinant plasmid was expressed in E. coli by IPTG induction. The fusion protein possessed both ST and LTB antigenicity, as well as it had lost the biological toxicity of ST or LT toxin. |
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| Keywords: | ETEC Enterotoxin Gene fusion and expression |
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