Separation of poly(A) RNA's synthesized by soybean embryos |
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| Authors: | Chandra G R; Abdul-Baki A |
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| Institution: | U. S. Department of Agriculture, ARS, Seed Research Laboratory, Beltsville Agricultural Research Center Beltsville, Maryland 20705, U.S.A. |
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| Abstract: | A stepwise elution procedure in which the MAK column with 0.85M buffered NaCl is held in a stationary phase (2–3 psi,35?C, 1 hr) permits the separation of two tenaciously boundRNA fractions. Poly(A) RNA's elute with the ribosomal and tenaciouslybound RNA's. MAK fractions contain different percent of RNA'sthat bind to poly(dT) cellulose and have messenger RNA activity.The results suggest that the soybean embryonic tissue synthesized,at least, four poly(A) RNA's. (Received August 17, 1976; ) |
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