Chromosomal mapping and nucleotide sequence of a human DNA autonomously replicating sequence |
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| Authors: | M Aguinaga C Sharan D N Singh D Kegler K A Siddiqui M S Valenzuela |
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| Institution: | Division of Biomedical Sciences, Meharry Medical College, Nashville, Tennessee 37208. |
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| Abstract: | A 1.1-kb human DNA fragment (ARSH1) capable of functioning as a putative origin of replication in yeast cells has been characterized both by in situ hybridization to human metaphase chromosomes and by DNA sequencing. Our hybridization studies show a preferential localization of ARSH1 in chromosome regions 1p34-36 and 2q34-37. DNA sequence analysis indicates that in addition to the consensus sequence required for ARS function in yeast cells, nuclear matrix-associated DNA motifs are also present in the 1.1-kb fragment. These results suggest that ARSH1 sequences may serve as points of anchorage to the nuclear matrix for chromosomes 1 and 2. |
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