Fusion of reconstituted influenza virus envelopes with liposomes mediated by streptavidin/biotin interactions |
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Authors: | Pieter Schoen Lee Leserman Jan Wilschut |
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Institution: | aGroningen Utrecht Institute for Drug Exploration (GUIDE), Department of Physiological Chemistry, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands;bCentre d'Immunologie, Institut National de la Santé et de la Recherche Médicale-Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille Cedex 9, France |
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Abstract: | Reconstituted influenza virus envelopes (virosomes) containing the viral hemagglutinin (HA) represent an efficient fusogenic cellular delivery system. By interaction of HA with its natural receptors, sialylated lipids (gangliosides) or proteins, virosomes bind to cells and, following endocytic uptake, deliver their contents to the cytosol through fusion from within acidic endosomes. Here, we show that binding to sialic acid is not necessary for fusion. In the presence of streptavidin, virosomes containing a biotinylated lipid fused with liposomes lacking sialic acid if these liposomes also had a biotinylated lipid in their membranes. Moreover, fusion characteristics corresponded well with fusion of virosomes with ganglioside-containing liposomes. |
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Keywords: | Influenza virus Hemagglutinin Virosome Membrane fusion Targeting Cellular delivery Carrier system |
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