Isolation and characterization of the gene encoding a manganese peroxidase from <Emphasis Type="Italic">Lentinula edodes</Emphasis> |
| |
Authors: | Masaru Nagai Yuichi Sakamoto Keiko Nakade Toshitsugu Sato |
| |
Institution: | (1) Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami Iwate, 024-0003, Japan;(2) Present address: Institute for Environmental Sciences, Japan |
| |
Abstract: | A genomic DNA sequence and cDNA encoding a putative manganese peroxidase were isolated from the white-rot basidiomycete Lentinula edodes. The gene, called lemnp1, consists of a 1985-bp open reading frame interrupted by 16 introns and was flanked by an upstream region having putative
CAAT, TATA, and heat shock elements and by a downstream region having polyadenylation signals. The lemnp1 gene encodes a protein of 364 amino acids that shows high sequence homology to manganese peroxidases of other basidiomycetes.
The deduced N-terminal amino acid sequence is different from the L. edodes manganese peroxidase reported previously. |
| |
Keywords: | Bioremediation Gene sequence Lentinula edodes Manganese peroxidase |
本文献已被 ScienceDirect SpringerLink 等数据库收录! |