Purification of monoiodinated vasointestinal peptide (M125I-VIP) by high pressure liquid chromatography (HPLC) |
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| Authors: | J.C. Marie C. Boissard G. Rosselin |
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| Affiliation: | Unité de Recherche de Diabétologie et d''Etudes Radio-Immunologiques des Hormones Protéiques U.55 (INSERM) Hôpital Saint-Antoine, 184, rue du Faubourg Saint-Antoine, 75571 Paris Cedex 12, France |
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| Abstract: | ![]()
In our developed reverse phase high performance liquid chromatography, four forms of M125I-VIP have been isolated free from unlabled VIP and other iodinated components. The quicker eluting M125I-VIP forms (oxidised and reduced) have a consistently and significantly low non-specific binding with specific target cells of VIP (HT-29) as compared to the late eluting forms of VIP. The retention time is considerably increased when the molecule of VIP is fully iodinated. |
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| Keywords: | Vasointestinal peptide (VIP) High performance liquid chromatography (HPLC) Human colon carcinoma cells (HT-29) |
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