Methods for the in vivo and in vitro analysis of [Het-s] prion infectivity |
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Authors: | Benkemoun Laura Sabaté Raimon Malato Laurent Dos Reis Suzana Dalstra Henk Saupe Sven J Maddelein Marie-Lise |
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Institution: | Laboratoire de Génétique Moléculaire des Champignons, IBGC UMR CNRS 5095, Université de Bordeaux 2, Bordeaux, France. |
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Abstract: | Prions have been described in mammals and fungi. The Het-s] infectious genetic element of the filamentous fungus Podospora anserina is the prion form of the HET-s protein. This protein is involved in the control of a cell death reaction termed heterokaryon incompatibility. The infectious form of HET-s corresponds to a self-perpetuating amyloid. The purpose of the present paper is to describe the techniques that can be used to analyse Het-s] prion propagation in vivo and HET-s amyloid aggregation in vitro. In addition, we report several methods that can be used to infect Podospora with recombinant HET-s amyloid. |
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