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| 黑曲霉mnn9基因缺失株的构建及其功能分析 | |
| 引用本文： | 王永超,秦浚川,王敖全,唐国敏,王华明.黑曲霉mnn9基因缺失株的构建及其功能分析[J].菌物学报,2006,25(1):70-76. |
| 作者姓名： | 王永超 秦浚川 王敖全 唐国敏 王华明 |
| 作者单位： | 1. 南京大学生命科学院,南京,210093 2. 中国科学院微生物研究所真菌和地衣系统学开放实验室,北京,100080 3. Genencor International Incorporation,California 94304,USA |
| 基金项目： | Joint Project granted by Genencor International Inc. of USA |
| 摘 要： | 本研究通过分析比较黑曲霉基因组与酿酒酵母基因组序列同源性,分离鉴定了黑曲霉mnn9基因。通过同源重组,在黑曲霉GICC2773(ΔAP4:pGPT-laccase)菌株中敲除了mnn9基因。该黑曲霉mnn9基因缺失使外源蛋白漆酶的分泌表达提高了14%,内源蛋白葡萄糖淀粉酶的分泌表达则降低了4%。 |
| 关 键 词： | 丝状真菌 同源重组 外源蛋白分泌 |
| 文章编号： | 1672-6472（2006）01-0070-0076 |
| 收稿时间： | 2005-10-28 |
| 修稿时间： | 2005-12-06 |
Construction and functional analysis of the mnn9 gene disruption mutant in Aspergillus niger |
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| Authors: | WANG Yong-Chao QIN Jun-Chuan WANG Ao-Quan TANG Guo-Min WANG Hua-Ming |
| Institution: | 1.Institute of Life Sciences, Nanjing University, Nanjing 210093, China; 2.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China; 3.Genencor International Inc. California 94304, USA |
| Abstract: | Here we use a comparative genomics approach to isolate mnn9 gene from Aspergillus niger which share high homology with S. cerevisiae mnn9 encoding mannosyltransferase responsible for the majority of the a-1,6-polymerizing activity of the mannoprotein . By double crossover, mnn9 gene in Aspergillus niger GICC2773 (ΔAP4: pGPT-laccase) strain was knocked out. Like all members of the S. cerevisiae mnn9 disruption mutant, in Aspergillus niger the mnn9 gene disruption mutant enhances heterologous protein laccase production by about 14% as compared with the parental strains, but decreases homologous protein glucoamylase production by about 4% |
| Keywords: | Hyphomycetous fungi homologous heterologous protein secretion |
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