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Analysis of the complete plastid genome of the unicellular red alga Porphyridium purpureum |
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| Authors: | Naoyuki Tajima Shusei Sato Fumito Maruyama Ken Kurokawa Hiroyuki Ohta Satoshi Tabata Kohsuke Sekine Takashi Moriyama Naoki Sato |
| Institution: | 1. Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo, 153-8902, Japan 2. JST, CREST, K’s Gobancho, 7 Gobancho, Chiyoda-ku, Tokyo, 102-0076, Japan 3. Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, 980-8577, Japan 4. Section of Bacterial Pathogenesis, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-ku, Tokyo, 113-8510, Japan 5. Earth-Life Science Institute, Tokyo Institute of Technology, 2-12-1-E3-10, Ookayama, Meguro-ku, Tokyo, 152-8550, Japan 6. Center for Biological Resources and Informatics, Tokyo Institute of Technology, 4259-B65 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan 7. Kazusa DNA Research Institute, Kazusa-Kamatari 2-6-7, Kisarazu, Chiba Prefecture, 292-0818, Japan |
| Abstract: | We determined the complete nucleotide sequence of the plastid genome of the unicellular marine red alga Porphyridium purpureum strain NIES 2140, belonging to the unsequenced class Porphyridiophyceae. The genome is a circular DNA composed of 217,694 bp with the GC content of 30.3 %. Twenty-nine of the 224 protein-coding genes contain one or multiple intron(s). A group I intron was found in the rpl28 gene, whereas the other introns were group II introns. The P. purpureum plastid genome has one non-coding RNA (ncRNA) gene, 29 tRNA genes and two nonidentical ribosomal RNA operons. One rRNA operon has a tRNAAla(UGC) gene between the rrs and the rrl genes, whereas another has a tRNAIle(GAU) gene. Phylogenetic analyses suggest that the plastids of Heterokontophyta, Cryptophyta and Haptophyta originated from the subphylum Rhodophytina. The order of the genes in the ribosomal protein cluster of the P. purpureum plastid genome differs from that of other Rhodophyta and Chromalveolata. These results suggest that a large-scale rearrangement occurred in the plastid genome of P. purpureum after its separation from other Rhodophyta. |
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