|
|||||
|
|
Structure of N-glycans on the S3- and S6-stylar self-incompatibility ribonucleases of Nicotiana alata |
|
| Authors: | Oxley David; Munro Sharon LA; Craik David J; Bacic Antony |
| Institution: | Plant Cell Biology Research Centre, School of Botany, University of Melbourne Parkville, Victoria 3052, Australia 1Victorian College of Pharmacy Parkville, Victoria 3052, Australia 2Center for Drug Design and Development, University of Queensland St Lucia, Queensland 4072, Australia |
| Abstract: | Self-incompatibility is a mechanism developed by many plantsto prevent inbreeding. The products of the selfincompatibility(S)-locus in the styles of solanaceous plants are a series ofglycoproteins with ribonuclease activity. In this study, wereport on the N-glycans from the stylar selfincompatibilityS3- and S6-ribonucleases of Nicotiana alata, which were enzymicallyreleased and fractionated by high-pH anion-exchange HPLC. Atotal of 14 N-glycans were identified and characterized by acombination of electrospray-ionization mass-spectrometry, 1H-NMRspectroscopy, chemical degradation, and methylation analyses.This pattern of N-glycosylation is much more complex than thatpreviously found on the N.alata S1- and S2-RNases each of whichcontained only four N-glycans. N-glycan Nicotiana alata ribonuclease selfincompatibility |
| Keywords: | |
| 本文献已被 Oxford 等数据库收录！ | |