Genetic analysis of variation in micropropagated plants of Melia azedarach L. |
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Authors: | S E Olmos G Lavia M Di Renzo L Mroginski V Echenique |
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Institution: | (1) Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto, Cabral 2131, 3400 Corrientes, Argentina;(2) Faculiad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Agencia n 3, 5800 Río Cuarto, Argentina;(3) CONICET, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca Buenos Aires, Argentina;(4) Departmento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca Buenos Aires, Argentina |
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Abstract: | Summary Plants were regenerated by shoot multiplication from four clones of Melia azedarach L. during 12 mo. of subculturing. One hundred and one of these plants were examined by randomly amplified polymorphic DNA
analysis. All regenerated plants showed at least one polymorphism. However, no chromosome number alterations were observed.
The pattern of variation obtained by principal coordinated analysis showed a random distribution of variation among regenerated
plants and their controls, indicating that genetic alterations were not cumulative during in vitro culture. Similar results were found using Shannon's index, which revealed that 50% of the observed diversity resided among
plants coming from the same subculture generation. This high intraclonal variation does not provide a clear scenario for predicting
the amount of culture time required to preserve genetic fidelity in commercially micropropagated M. azedarach plants. Our work suggests that other mechanisms, such as chimerism, contribute to intraclonal heterogeneity in vitro. |
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Keywords: | somaclonal variation RAPD diversity analysis chromosome counting |
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