Quantitative proteomics by amino acid labeling in C. elegans |
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Authors: | Fredens Julius Engholm-Keller Kasper Giessing Anders Pultz Dennis Larsen Martin Røssel Højrup Peter Møller-Jensen Jakob Færgeman Nils J |
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Institution: | Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark. |
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Abstract: | We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology. |
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