| 农杆菌介导单子叶植物遗传转化问题与对策 |
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| 引用本文： | 魏开发,刘逸萍,林子英,杨雅芳,张泽宏,贾文锁.农杆菌介导单子叶植物遗传转化问题与对策[J].植物学通报,2008,25(4):491-496. |
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| 作者姓名： | 魏开发 刘逸萍 林子英 杨雅芳 张泽宏 贾文锁 |
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| 作者单位： | 1. 漳州师范学院生物系,漳州,363000
2. 中国农业大学农学与生物技术学院,北京,100094 |
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| 摘 要： | 尽管十几年来农杆菌介导的单子叶植物遗传转化研究取得了较大的进步，但仍存在着基因型限制、转化率不高和外源基因表达活性低等问题。本文综述了近几年来此项研究在感受态细胞选择与调节、预培养及共培养体系优化、转化子的筛选及外源基因表达调控等方面的主要进展。
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| 关 键 词： | 农杆菌 遗传转化 单子叶植物 |
Problems and Solutions in Agrobacterium tumefaciens-mediated Genetic Transformation of Monocotyledons |
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| Kaifa Wei,Yiping Liu,Ziying Lin,Yafang Yang,Zehong Zhang,Wensuo Jia.Problems and Solutions in Agrobacterium tumefaciens-mediated Genetic Transformation of Monocotyledons[J].Chinese Bulletin of Botany,2008,25(4):491-496. |
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| Authors: | Kaifa Wei Yiping Liu Ziying Lin Yafang Yang Zehong Zhang Wensuo Jia |
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| Institution: | Kaifa Wei, Yiping Liu, Ziying Lin, Yafang Yang, Zehong Zhang, Wensuo Jia( 1.Department of Biotechnology, Zhangzhou Normal College, Zhangzhou 363000, China; 2.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China) |
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| Abstract: | Many transgenic plants have been obtained through the Agrobacterium-mediated transformation for monocotyledons. However, transformation of monocotyledons plants has been constrained by factors affecting transformation efficiency such as gene type, callus induction and differentiation, and foreign gene expression. This review discusses some technological details and problems, and reviews the progresses, including selection and management of competent cells, optimization of preculture and coculture, selection, foreign gene expression and regulation. |
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| Keywords: | Agrobacterium tumefaciens genetic transformation monocotyledons |
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