| 25S rDNA在杨属植物染色体上的定位 |
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| 引用本文： | 董凤平,韩素英,张守攻,齐力旺,刘博,李秀兰,陈成彬.25S rDNA在杨属植物染色体上的定位[J].云南植物研究,2007,29(4):423-428. |
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| 作者姓名： | 董凤平 韩素英 张守攻 齐力旺 刘博 李秀兰 陈成彬 |
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| 作者单位： | 1. 南开大学生命科学学院,天津,300071
2. 中国林业科学院林业研究所细胞生物学实验室,北京,100091 |
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| 基金项目： | 国家自然科学基金;天津市自然科学基金 |
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| 摘 要： | 利用荧光原位杂交技术对杨属(Populus)植物五个组中二倍体(2n=2x=38)代表种:毛白杨(P.tomentosa)、箭杆杨(P.nigravar.thevestina)、大叶杨(P.lasiocarpa)、小青杨(P.pseudo-simonii)、胡杨(P.euphratica);以及所发现的白杨组和黑杨组天然三倍体(2n=3x=57):毛白杨(P.tomentosa)、武黑1号(P.euramericana cv.Wuhei-1)进行了25S rDNA的染色体定位。二倍体毛白杨、箭杆杨、小青杨和大叶杨都具有4个25S rDNA位点,而胡杨只有2个较大的25S rDNA定位于1对小的染色体上,白杨和黑杨天然三倍体的两个种各有6个25S rDNA位点。同时作者还将杨属植物25S rDNA的分布变化与常规核型分析结果进行了比较。
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| 关 键 词： | 杨属 荧光原位杂交 25S rDNA定位 |
| 文章编号： | 0253-2700（2007）04-423-06 |
| 修稿时间： | 2006-12-222007-02-25 |
Physical Mapping of 25S rDNA on Metaphase Chromosomes of Populus (Salicaceae) in Five Sections by Fluorescence in Situ Hybridization |
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| DONG Feng-Ping,HAN Su-Ying,ZHANG Shou-Gong,QI Li-Wang,LIU Bo,LI Xiu-Lan,CHEN Cheng-Bin.Physical Mapping of 25S rDNA on Metaphase Chromosomes of Populus (Salicaceae) in Five Sections by Fluorescence in Situ Hybridization[J].Acta Botanica Yunnanica,2007,29(4):423-428. |
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| Authors: | DONG Feng-Ping HAN Su-Ying ZHANG Shou-Gong QI Li-Wang LIU Bo LI Xiu-Lan CHEN Cheng-Bin |
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| Institution: | 1.College of Life Science, Nankai University, Tianjin 300071, China; 2.Laboratory of Cell Biology, the Research Institute of Forestry, the Chinese Academy of Forestry, Beijing 100091, China |
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| Abstract: | This study examined the physical localizations of the 25S rDNA on the mitotic metaphase chromosomes of seven species in the five sections of Populus,using fluorescence in situ hybridization(FISH).Four 25 rDNA loci were detected on the chromosomes of five wild diploid species(2n=2x=38),P.tomentosa,P.nigra var.thevestina,P.pseudo-simonii,P.lasiocarpa and P.euphratica.Six 25 rDNA loci were detected on the chromosomes of two wild triploid species(2n=3x=57),P.tomentosa in section Leuce and P.euramericana cv.Wuhei-1 in section Aigeiros.While only one pair of 25 rDNA loci located at the smallest chromosome was detected in P.euphratica chromosomes.Coupled with the approach of common karoytype analysis,this article also discusses the variations of 25S rDNA distribution on Populus chromosomes. |
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| Keywords: | Populus Fluorescence in situ hybridization(FISH) 25S rDNA loci |
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