Physiological and molecular characterization of salt response of <Emphasis Type="Italic">Arabidopsis thaliana NOK2</Emphasis> ecotype |
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Authors: | Rym Kaddour Sabah M’rah Najoua Karray-Bouraoui Cécile Lambert Pierre Berthomieu Mokhtar Lachaâl |
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Institution: | 1.Physiologie et Biochimie de la Tolérance au Sel des Plantes, Faculté des Sciences de Tunis,Tunis,Tunisia;2.Biochimie & Physiologie Moléculaire des Plantes,SupAgro/INRA,Montpellier Cedex 1,France |
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Abstract: | Arabidopsis thaliana is a glycophyte capable to tolerate mild salinity. Although salt sensitivity of this species, a variability of this characteristic
was revealed between different ecotypes. This study presents the physiological and molecular characteristics of salt response
of two ecotypes, NOK2 and Columbia (Col). Seedlings were cultivated in hydroponics in the presence of 0 or 50 mM NaCl during 25 days. Rosette leaf samples were collected
after 19, 22, and 25 days for determination of physiological parameters, and after 18 days for study of DNA polymorphism.
Salt treatment decreased rosette dry matter, leaf number, leaf hydration, and leaf surface area. All these effects were significantly
more visible in Col than in NOK2. Moreover, the NOK2 leaves accumulated less Na+ and more K+ than those of Col. DNA polymorphism between the two ecotypes was analyzed with codominant molecular markers based on PCR amplification, namely,
microsatellites, cleaved amplified polymorphism sequence (CAPS), and single nucleotide polymorphism markers (SNP). Among the
35 tested markers, 17 showed a clear polymorphism and were distributed on the five Arabidopsis chromosomes ending with a genetic map construction. These results could play an important role in the future establishment
of cartography of candidate gene controlling the K+/Na+ selectivity of ion transport in leaves, a component of plant salt tolerance. |
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