酶的分子定向进化及其应用

酶的分子定向进化是20世纪90年代初兴起的一种蛋白质工程的新策略,是一种在生物体外模拟自然进化过程的、具有一定目的性的快速改造蛋A质的方法.该方法引起了生物催化技术领域的又一次革命.目前分子定向进化技术已被广泛应用于工业、农业及制药业等的相关领域.本文详细综述了酶的分子定向进化的概念、过程、基本策略及其核心技术,并着重介绍了酶的分子定向进化技术在提高酶的活力、稳定性、底物特异性和对映体选择性等几方面的应用及取得的相关成果.

Directed Molecular Evolution of Enzyme and its Application

Directed molecular evolution of enzyme,originated in the beginning of the nineties of the last century,was a new strategy of protein engineering,which could rapidly modify proteins for specific purpose through imitating extemally natural evolutions,as well as it has arisen a revolution in biocatalyst field.Nowadays,this technology has been widely applied in various fields including chemical engineering,agriculture and pharmaceutical industry.This paper elaborates on the concept of directed molecular evolution,procedure,basic strategies,and its core technology,then presents emphatically its application and achievements in improving enzyme activities,stability,substrate specificity,and antipode selectivity and so on.