Degenerative and apoptotic events at retinal and optic nerve level after experimental induction of ocular hypertension |
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Authors: | Nicola Calandrella Gianfranco Scarsella Nicola Pescosolido Gianfranco Risuleo |
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Institution: | (1) Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma “La Sapienza”, Piazzale Aldo Moro, 5, 00185 Rome, Italy;(2) Dipartimento di Scienze dell’ invecchiamento Sezione di Oftalmologia, Università di Roma “La Sapienza”, Piazzale Aldo Moro, 5, 00185 Rome, Italy;(3) Dipartimento di Genetica e Biologia Molecolare, Università di Roma “La Sapienza”, Piazzale Aldo Moro, 5, 00185 Rome, Italy |
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Abstract: | Ocular hypertension is a symptom of a glaucomatous condition characterized by a severe vision decrease. Blindness caused by
the apoptotic death of the retinal ganglion cells and of the astrocytes of the optic nerve may eventually result. Experimental
hypertension was induced by inoculation of methylcellulose in the anterior chamber. Chromatin staining, TUNEL assay, and inter-nucleosomal
DNA fragmentation observed in retina and optic nerve strongly suggest that hypertension causes apoptosis. Immunolocalization
of the fibrillary acidic glial protein, specific of cell stress, and caspase-3 in the same tissues, further support this mode
of cell death. Activation of the ubiquitin dependant proteolytic system was also observed. Protection from apoptosis exerted
by administration of the peroxide scavenger trolox, suggests that the apoptotic pathway is activated by an oxidative stress.
The data presented here show that the experimental hypertensive insult induces degenerative and apoptotic events comparable
to those observed in human glaucoma. |
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Keywords: | Experimental hypertension Glaucoma Retina Optic nerve Apoptosis |
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