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6-苄基腺嘌呤对平菇和香菇菌丝体4种胞外酶活性的影响
引用本文:邱昌恩,邵锦震,陈少英,姚发兴.6-苄基腺嘌呤对平菇和香菇菌丝体4种胞外酶活性的影响[J].武汉植物学研究,2002,20(3):233-235.
作者姓名:邱昌恩  邵锦震  陈少英  姚发兴
作者单位:湖北师范学院生物系,湖北黄石,435002
摘    要:在马铃薯液体培养基中,通过活加不同剂量的6-苄基腺嘌呤(6-BA),测定了平菇和香菇菌丝体4种胞外酶活性的变化。结果表明,低浓度的外源6-BA对平菇、香菇菌丝体4种胞外酶活性都有促进作用,高浓度时则逐渐抑制酶活性。其中,培养液浓度为1.5mg/100mL时,它们扔包外粉酶(Amy)、羧甲基纤维素酶(CMC)、过氧化氢酶(CAT)的活性最高;而它们的胞外蛋白酶的最适浓度为0.5mg/100mL。

关 键 词:6-苄基腺嘌呤  平菇  香菇  胞外酶  酶活性
文章编号:1000-470(2002)03-0233-03
收稿时间:2001-8-27
修稿时间:2002-4-27

The Effect of 6-Benzyl Aminopurine on the Activities of Four Extracellular Enzymes in Plurotus ostreatus and Lentinula edodes Mycelium
QIU Chang En,SHAO Jin Zhen,CHEN Shao Ying,YAO Fa Xin.The Effect of 6-Benzyl Aminopurine on the Activities of Four Extracellular Enzymes in Plurotus ostreatus and Lentinula edodes Mycelium[J].Journal of Wuhan Botanical Research,2002,20(3):233-235.
Authors:QIU Chang En  SHAO Jin Zhen  CHEN Shao Ying  YAO Fa Xin
Institution:Department of Biology, Hubei Normal University, Huangshi, Hubei 435002, China
Abstract:Four extracellular enzyme activities in Pleurotus ostreatus and Lentinula edodes mycelium were determined by adding to different dosage additional 6 benzyl aminopurine (6 BA) in potato liquid medium. The results showed that low concentration of 6 BA promoted their activities and high concentration inhibited their activities. For Pleurotus ostreatus and Lentinula edodes, the optimum concentration of 6 BA to activate their extracellular amylase, carboxymenthel cellulase and catalase was the same which was 1 5 mg/100 mL culture solution. But, the optimum concentration for their extracellular protease was 0 5 mg/100 mL culture solution.
Keywords:benzyl aminopurine  Pleurotus ostreatus  Lentinula edodes  Extracellular enzymes  Enzyme activity
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