High-density Linkage Map of Cultivated Allotetraploid Cotton Based on SSR, TRAP, SRAP and AFLP Markers |
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| Authors: | Jiwen Yu Shuxun Yu Cairui Lu Wu Wang Shuli Fan Meizhen Song Zhongxu Lin Xianlong Zhang Jinfa Zhang |
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| Affiliation: | (National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University;, Wuhan 430070, China;Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture;, Anyang 455004, China;China Cotton Research Institute, Chinese Academy of Agricultural Science;, Anyang 455004, China;Department of Plant and Environmental Sciences, New Mexico State University;, Las Cruces, NM 88003, USA) |
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| Abstract: | A high-density linkage map was constructed for an F2 population derived from an interspecific cross of cultivated allotetraploid species between Gossyplum hirsutum L. and G. barbadense L. A total of 186 F2 individuals from the interspecific cross of "CRI 36 × Hai 7124" were genotyped at 1 252 polymorphic loci including a novel marker system,target region amplification polymorphism (TRAP). The map consists of 1 097 markers, including 697 simple sequence repeats (SSRs), 171 TRAPs, 129 sequence-related amplified polymorphisms, 98 amplified fragment length polymorphisms, and two morphological markers, and spanned 4 536.7 cM with an average genetic distance of 4.1 cM per marker. Using 45 duplicated SSR loci among chromosomes, 11 of the 13 pairs of homologous chromosomes were identified in tetraplold cotton. This map will provide an essential resource for high resolution mapping of quantitative trait loci and molecular breeding in cotton. |
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| Keywords: | cotton linkage map target region amplification polymorphism |
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