Xanthophyll Cycle and Inactivation of Photosystem Ⅱ Reaction Centers Alleviating Reducing Pressure to Photosystem Ⅰ in Morning Glory Leaves under Short-term High Irradiance |
| |
作者姓名: | Xin-Guo Li Jian-Yong Li Jin-Ping Zhao Ping-Li Xu Qi-Wei He. |
| |
作者单位: | [1]High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji' nan 250100, China [2]Ji' nan Entry-exit Inspection Quarantine Bureau of the People's Republic of China, Ji' nan 250100, China [3]Vegetable Institute, Shandong Academy of Agricultural Sciences, Ji' nan 250100, China |
| |
基金项目: | Supported by the Natural Science Foundation of China (30571126, 30671242), the Scientific Research Encouragement Foundation for 0utstanding Young and Middle Scientists of Shandong Province (2005BS06003). |
| |
摘 要: | Under 30-min high irradiance (1500μmol m^-2 s^-1), the roles of the xanthophyll cycle and D1 protein turnover were investigated through chlorophyll fluorescence parameters in morning glory (Ipomoea setosa) leaves, which were dipped into water, dithiothreitol (DTT) and lincomycin (LM), respectively. During the stress, both the xanthophyll cycle and D1 protein turnover could protect PSI from photoinhibition. In DTT leaves, non-photochemical quenching (NPQ) was inhibited greatly and the oxidation level of P700 (P700^+) was the lowest one. However, the maximal photochemical efficiency of PSII (Fv/Fm) in DTT leaves was higher than that of LM leaves and was lower than that of control leaves. These results suggested that PSI was more sensitive to the loss of the xanthophyll cycle than PSII under high irradiance. In LM leaves, NPQ was partly inhibited, Fv/Fm was the lowest one among three treatments under high irradiance and P700^+ was at a similar level as that of control leaves. These results implied that inactivation of PSII reaction centers could protect PSI from further photoinhibition. Additionally, the lowest of the number of active reaction centers to one inactive reaction center for a PSII cross-section (RC/CSo), maximal trapping rate in a PSll cross-section (TRo/CSo), electron transport in a PSll cross-section (ETo/CSo) and the highest of 1-qP in LM leaves further indicated that severe photoinhibition of PSII in LM leaves was mainly induced by inactivation of PSII reaction centers, which limited electrons transporting to PSh However, relative to the LM leaves the higher level of RC/CSo, TRo/CSo, Fv/Fm and the lower level of 1-qP in DTT leaves indicated that PSI photoinhibition was mainly induced by the electron accumulation at the PSI acceptor side, which induced the decrease of P700^+ under high irradiance.
|
关 键 词: | D1蛋白质 高辐照度 黄色质周期 光合体系 |
修稿时间: | 2006-06-212006-09-16 |
Xanthophyll Cycle and Inactivation of Photosystem II Reaction Centers Alleviating Reducing Pressure to Photosystem I in Morning Glory Leaves under Short-term High Irradiance |
| |
Authors: | Xin-Guo Li Jian-Yong Li Jin-Ping Zhao Ping-Li Xu Qi-Wei He |
| |
Institution: | ( High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji' nan 250100, China;;Ji' nan Entry-exit Inspection Quarantine Bureau of the People' s Republic of China, Ji' nan 250100, China;;Vegetable Institute, Shandong Academy of Agricultural Sciences, Ji' nan 250100, China;) |
| |
Abstract: | Under 30‐min high irradiance (1500 μmol m?2 s?1), the roles of the xanthophyll cycle and D1 protein turnover were investigated through chlorophyll fluorescence parameters in morning glory (Ipomoea setosa) leaves, which were dipped into water, dithiothreitol (DTT) and lincomycin (LM), respectively. During the stress, both the xanthophyll cycle and D1 protein turnover could protect PSI from photoinhibition. In DTT leaves, non‐photochemical quenching (NPQ) was inhibited greatly and the oxidation level of P700 (P700+) was the lowest one. However, the maximal photochemical efficiency of PSII (Fv/Fm) in DTT leaves was higher than that of LM leaves and was lower than that of control leaves. These results suggested that PSI was more sensitive to the loss of the xanthophyll cycle than PSII under high irradiance. In LM leaves, NPQ was partly inhibited, Fv/Fm was the lowest one among three treatments under high irradiance and P700+ was at a similar level as that of control leaves. These results implied that inactivation of PSII reaction centers could protect PSI from further photoinhibition. Additionally, the lowest of the number of active reaction centers to one inactive reaction center for a PSII cross‐section (RC/CSo), maximal trapping rate in a PSII cross‐section (TRo/CSo), electron transport in a PSII cross‐section (ETo/CSo) and the highest of 1‐qP in LM leaves further indicated that severe photoinhibition of PSII in LM leaves was mainly induced by inactivation of PSII reaction centers, which limited electrons transporting to PSI. However, relative to the LM leaves the higher level of RC/CSo, TRo/CSo, Fv/Fm and the lower level of 1‐qP in DTT leaves indicated that PSI photoinhibition was mainly induced by the electron accumulation at the PSI acceptor side, which induced the decrease of P700+ under high irradiance. |
| |
Keywords: | D1 protein high irradiance morning glory photoinhibition xanthophyll cycle |
本文献已被 维普 等数据库收录! |
|