Electron Tomography in Plant Cell Biology |
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Authors: | Thomas J Haas Marisa S Otegui |
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Institution: | ( Department of Botany, University of Wisconsin-Madison, Madison, WI 53706, USA;) |
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Abstract: | This review focuses on the contribution of electron tomography-based techniques to our understanding of cellular processes in plant cells. Electron microscopy techniques have evolved to provide better three-dimensional resolution and improved preservation of the subcellular components. In particular, the combination of cryofixation/freeze substitution and electron tomography have allowed plant cell biologists to image organelles and macromolecular complexes in their native cellular context with unprecedented three-dimensional resolution (4-7 nm). Until now, electron tomography has been applied in plant cell biology for the study of cytokinesis, Golgi structure and trafficking, formation of plant endosome/prevacuolar compartments, and organization of photosynthetic membranes. We discuss in this review the new insights that these tomographic studies have brought to the plant biology field. |
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Keywords: | cell structure electron tomography high-pressure freezing/freeze substitution |
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