Evidence for the presence of lipopolysaccharide in Treponema phagedenis (biotype Reiterii) but not in Treponema pallidum (Nichols) |
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Authors: | MJ Bailey CW Penn A Cockayne |
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Institution: | Department of Microbiology, University of Birmingham, South-West Campus, Birmingham, B15 2TT, U.K. |
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Abstract: | Abstract Immunoblotting profiles of whole or protease-K-digested organisms with homologous antisera demonstrated the presence of a characteristic ladder pattern of smooth LPS in Treponema phagedenis . Periodic acid silver staining of SDS-PAGE gels confirmed these findings. However, when heterologous or homologous serum was reacted with Treponema pallidum , no such pattern or cross-reactions were observed. The significance of apparent absence of LPS in T. pallidum is discussed. |
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Keywords: | Western blotting syphilis antigenic SDS-PAGE |
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