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RASA contains a polymorphic microsatellite and maps to bovine syntenic group U22 on Chromosome 7q2.4-qter |
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| Authors: | André Eggen Sabina Solinas-Toldo Allan B. Dietz James E. Womack Gerald Stranzinger Ruedi Fries |
| Affiliation: | (1) Department of Animal Science, Swiss Federal Institute of Technology, Zurich, Switzerland;(2) Department of Veterinary Pathology, Texas A&M University, 77843 College Station, Texas, USA;(3) Present address: National Animal Disease Center, 50010 Ames, Iowa, USA |
| Abstract: | The bovine gene for the p21ras protein activator (RASA) includes in its 5  untranslated region a (TG)n repeat. Analysis of this (TG)n repeat by PCR amplification of genomic DNA revealed a four-allele polymorphism. A cDNA probe was used to assign RASA to the region 2.4-qter of bovine Chromosome (Chr) 7 by in situ hybridization. PCR analysis of a panel of somatic hybrid lines allowed the assignment of RASA to the unassigned syntenic group 22 (U22) and thus localizes U22 on Chr 7. |
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